ABSTRACT
This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health.
Subject(s)
Humans , Animals , Campylobacter jejuni/isolation & purification , Campylobacter jejuni/physiology , Eggs/microbiology , Microbial ViabilityABSTRACT
A mastite ocupa lugar de destaque entre as doenças que acometem o rebanho leiteiro, em virtude de problemas econômicos e de saúde pública. Staphylococcus spp são os agentes infecciosos mais envolvidos na etiologia da doença. Staphylococcus spp resistentes à oxacilina, isolados de amostras de leite provenientes de animais com histórico de mastite recorrente, foram analisadas utilizando-se os testes de difusão em gel, ágar de triagem, concentração mínima inibitória (CMI) e pesquisa do gene mecA. De 134 amostras de leite analisadas, Staphylococcus spp foi isolado em 55,22% das amostras (74/134). O teste de difusão em disco demonstrou que a maioria dos isolados apresentaram multiresistência, sendo 51,35% (38/74) resistentes à oxacilina. Esse perfil foi confirmado em paralelo pela CMI e pelo ágar de triagem. A análise molecular demonstrou que 33,78% dos isolados (25/74) possuíam o gene mecA, sendo mais frequentemente isolado em Staphylococcus aureus e Staphylococcus coagulase negativa, com índices de 48% (12/25) e 32% (8/25),respectivamente, seguido de S. intermedius com 16% (4/25) e de S. hyicus com 4% (1/25). O presente estudo denota o grave problema associado à Staphylococcus aureus resistente à oxacilina, bem como no meio rural, o que justifica a dificuldade de tratamento e a recorrência da infecção.
Mastitis is of a major concern among the diseases that affect the dairy herd due to the economic problemsand public health concerns. Staphylococcus spp has been the infectious bacterium and the etiologic agentof this disease. The oxacillin resistance of Staphylococcus spp isolated from milk samples of animals witha history of recurrent mastitis was investigated. This study was performed by means of gel diffusion test,agar screening, minimum inhibitory concentration (MIC), and the mecA gene searching. Of 134 milksamples, Staphylococcus spp was isolated from 55.22% samples (74/134). By means of disk diffusion test,the majority of the isolates showed multiresistance, and 51.35% (38/74) were resistant to oxacillin. Thisprofile was confirmed in parallel by CMI and the agar screening. By molecular analysis 33.78% of isolates(25/74) revealed the mecA gene, being frequently isolated from Staphylococcus aureus and coagulase negativeStaphylococcus at rates of 48% (12/25) and 32% (8/25 ), respectively, and followed by S. intermedius in 16%(4 / 25) and S. hyicus in 4% (1/25). The serious problem associated with oxacillin-resistant Staphylococcusaureus was found this study, including in rural environment and these findings justifie the treatmentdifficulty and the recurrence of infection.